NMR service details
Bruker Avance 400 (DPX-400)
This spectrometer has a standard geometry 5mm 1H/13C/19F/31P probe. It can perform a wide variety of 1D and 2D experiments. With its sample changer and Icon-nmr, this machine is now used predominantly for undergraduate teaching.
Bruker Avance 400
This spectrometer has a standard geometry 5mm 1H/13C probe and is equipped with sample changer. It is open access using Icon-nmr. This spectrometer is used by the level 4 undergraduates and the majority of postgraduates in the Department.
Bruker Avance III HD 400
This spectrometer has a standard geometry 5mm BBFO probe with a single z-gradient and can observe 1H,13C,19F,31P and most NMR active isotopes in the periodic table. Experiments such as HH-COSY, JMOD, DEPTQ can be performed routinely. It is open access with sample changer and Icon-nmr. The vast majority of the routine Level 4 and postgraduate NMR samples are run on this spectrometer.
Bruker Avance III 400
This spectrometer has a standard geometry 5mm BBFO probe with a single z-gradient and can observe 1H,13C,19F,31P and most NMR active isotopes in the periodic table. The probe has been constructed to give maximum sensitivity to X-nuclei not 1H. It can perform a wide variety of 1D and 2D experiments and has variable temperature capabilities of ca. ± 100 °C.
Bruker Avance III HD 500
This spectrometer has three probes: a standard geometry 5mm BBFO probe, an inverse geometry 5mm VSP probe, and a 4mm CP/MAS probe. Both solution probes have a single z-gradient and can observe 1H and most NMR active isotopes in the periodic table. It can perform a wide variety of 1D and 2D experiments and has variable temperature capabilities of ca. ± 100 °C. The CP/MAS probe can observe 1H/19F on 1H channel and 14N-31P on BB channel. 4mm rotors, with a maximum spinning speed of 15 kHz, of different sample sizes (12uL, 50uL, and 80uL) are available.
There are many types of NMR experiment, the time and amount of compound required varies enormously. A simple 1H spectrum can be completed in minutes whereas a ROESY experiment on a weak sample may take all weekend. The following is just a very small selection.
Whilst the sample may be confidential it very important that all relevant information is noted on the NMR form such as 1H signal expected at 13 ppm or 13C signal at –35 ppm. Similarly it is important that the sample dissolves fully in the NMR solvent otherwise the spectrum relates not to the compound but to the compound and the soluble impurities.
Sample quantity depends upon the nucleus and experiment required:
These figures are only "ball-park" figures and it depends on molecular weight, symmetry, sample availability and solubility.
A wide range of deuterated NMR solvents is available. The cost of deuterated solvents varies enormously from a few pence a ml for chloroform-d to over £10 a ml for N,N-dimethylformamide-d7. Depending on the choice of solvent there may be a surcharge as shown in the table below:
If required, other solvents may be purchased.
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